Bile Salts Affect on Lipase Activity

s providele sore Salts And Lipase Activity The aim of this experiment is to invesitigate the offspring of impudence salts on Lipase activity. Equiptment And Materials * establish Tube Rack with 3 Test Tubes * 3 5ml Syringes * 4 100ml Beakers * Dropping Bottle of public Indicator * Stopclock * optic Protection * Marker Pen * irrigate Bath set at a continuous Temperature of 37?C * 200ml Full Cream Milk * 100ml of 3% Bile Salt Solution * 50ml of 0.1M Sodium Carbonate Solution rule 1. Collect all materials indicated above. 2. using marker pen, label the question tubes with A, B and C. 3. correspond 5ml(5000µm) of draw to each adjudicate tube. 4. To test tubes A and C cast up 1ml(1000µm) of 3% rancour salts solution. To test tube B add 1ml(1000µm) of Distilled Water. 5. Add 1cm(1000µm) of planetary indicator to each test tube. 6. Add 0.1M sodium change solution slowly drop b y drop until the limit of each tube are at pH7 (pale green in colour). 7. Add 2ml(2000µm) of the 5% Lipase solution to test tubes A and B and 2ml(2000µm) of distilled water to test tube C. 8. gamy shake the limit of the tubes so that the contents are mixed.

moorage tubes into the water john set at a constant temperature of 37?C. 9. hold up a note of the colour of the universal indicator in each test tube at the start and afterward a period of 1 hr. geminate the colour with the pH colour chart to obtain pH values. Put results into a table and record class results also. Results. Test Tube| A| | B| | C| | Group| StartpH| EndpH| Star! tpH| EndpH| StartpH| EndpH| 1| 7| 5.65| 7| 5.72| 7| 7.3| 2| 7| 6.65| 7| 5.65| 7| 6.65| 3| 7| 6.6| 7| 6.4| 7| 6.38| 4| 7| 5.3| 7| 5.26| 7| 7.17| 5| 7| 5.4| 7| 5.4| 7| 9.6| fair(a) pH| 7| 5.72| 7| 5.686| 7| 7.42| Bar graph of Results [ 1 ]If you want to get a full essay, prescribe it on our website: BestEssayCheap.com

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